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HEPH Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant HEPH.

  • WB - HEPH Antibody (monoclonal) (M01) AT2350a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
  • E - HEPH Antibody (monoclonal) (M01) AT2350a
    Detection limit for recombinant GST tagged HEPH is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BQS7
Other Accession NM_138737
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2D3
Calculated MW 130449 Da
Additional Information
Gene ID 9843
Other Names Hephaestin, 1---, HEPH, KIAA0698
Target/Specificity HEPH (NP_620074, 315 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHEPH Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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The protein encoded by this gene is similar to an iron transport protein found in mouse. The mouse protein is similar to ceruloplasmin, a serum multi-copper ferroxidase, and is thought to be a membrane-bound protein responsible for transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system. In mouse, defects in this gene can lead to severe microcytic anemia. Three transcript variants encoding different isoforms have been described for this gene.


Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection. Ucisik-Akkaya E, et al. Mol Hum Reprod, 2010 Oct. PMID 20587610.Human hephaestin expression is not limited to enterocytes of the gastrointestinal tract but is also found in the antrum, the enteric nervous system, and pancreatic {beta}-cells. Hudson DM, et al. Am J Physiol Gastrointest Liver Physiol, 2010 Mar. PMID 20019163.Decreased hephaestin expression and activity leads to decreased iron efflux from differentiated Caco2 cells. Chen H, et al. J Cell Biochem, 2009 Jul 1. PMID 19452451.Neither human hephaestin nor ceruloplasmin forms a stable complex with transferrin. Hudson DM, et al. J Cell Biochem, 2008 Apr 15. PMID 18022819.Colocalization of ferroportin-1 with hephaestin on the basolateral membrane of human intestinal absorptive cells. Han O, et al. J Cell Biochem, 2007 Jul 1. PMID 17486601.

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$ 315.00
Cat# AT2350a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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