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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HFE Antibody (monoclonal) (M01)   

HFE Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant HFE.

  • WB - HFE Antibody (monoclonal) (M01) AT2361a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.75 KDa) .
  • WB - HFE Antibody (monoclonal) (M01) AT2361a
    HFE monoclonal antibody (M01), clone 1G12. Western Blot analysis of HFE expression in A-431.
  • WB - HFE Antibody (monoclonal) (M01) AT2361a
    Western Blot analysis of HFE expression in transfected 293T cell line by HFE monoclonal antibody (M01), clone 1G12.

    Lane 1: HFE transfected lysate(40.1 KDa).
    Lane 2: Non-transfected lysate.
  • IF - HFE Antibody (monoclonal) (M01) AT2361a
    Immunofluorescence of monoclonal antibody to HFE on HeLa cell. [antibody concentration 10 ug/ml]
  • E - HFE Antibody (monoclonal) (M01) AT2361a
    Detection limit for recombinant GST tagged HFE is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q30201
Other Accession NM_000410
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1G12
Calculated MW 40108 Da
Additional Information
Gene ID 3077
Other Names Hereditary hemochromatosis protein, HLA-H, HFE, HLAH
Target/Specificity HFE (NP_000401, 115 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHFE Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.


1.Hemochromatosis Enhances Tumor Progression via Upregulation of Intracellular Iron in Head and Neck Cancer.Lenarduzzi M, Hui AB, Yue S, Ito E, Shi W, Williams J, Bruce J, Sakemura-Nakatsugawa N, Xu W, Schimmer A, Liu FFPLoS One. 2013 Aug 26;8(8):e74075. doi: 10.1371/journal.pone.0074075.

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$ 315.00
Cat# AT2361a
(40 western blots)
Availability: 7-10 days
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