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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IDH2 Antibody (monoclonal) (M01)   

IDH2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant IDH2.

  • WB - IDH2 Antibody (monoclonal) (M01) AT2479a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.52 KDa) .
  • WB - IDH2 Antibody (monoclonal) (M01) AT2479a
    Western Blot analysis of IDH2 expression in transfected 293T cell line by IDH2 monoclonal antibody (M01), clone 5F11.

    Lane 1: IDH2 transfected lysate(50.9 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - IDH2 Antibody (monoclonal) (M01) AT2479a
    Immunoperoxidase of monoclonal antibody to IDH2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
  • E - IDH2 Antibody (monoclonal) (M01) AT2479a
    Detection limit for recombinant GST tagged IDH2 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48735
Other Accession NM_002168
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 5F11
Calculated MW 50909 Da
Additional Information
Gene ID 3418
Other Names Isocitrate dehydrogenase [NADP], mitochondrial, IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2
Target/Specificity IDH2 (NP_002159, 354 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsIDH2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.


1.Altered global methylation and hydroxymethylation status in vulvar lichen sclerosus - further support for epigenetic mechanisms.Gambichler T, Terras S, Kreuter A, Skrygan MBr J Dermatol. 2013 Oct 27. doi: 10.1111/bjd.12702.

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$ 315.00
Cat# AT2479a
(40 western blots)
Availability: 7-10 days
Bulk Size
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