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IRAK1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant IRAK1.

  • WB - IRAK1 Antibody (monoclonal) (M01) AT2538a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (43.45 KDa) .
  • WB - IRAK1 Antibody (monoclonal) (M01) AT2538a
    Western Blot analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M01), clone 3F7.

    Lane 1: IRAK1 transfected lysate(68 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - IRAK1 Antibody (monoclonal) (M01) AT2538a
    Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
  • IF - IRAK1 Antibody (monoclonal) (M01) AT2538a
    Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - IRAK1 Antibody (monoclonal) (M01) AT2538a
    Immunoprecipitation of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.
  • E - IRAK1 Antibody (monoclonal) (M01) AT2538a
    Detection limit for recombinant GST tagged IRAK1 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51617
Other Accession BC054000
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3F7
Calculated MW 76537 Da
Additional Information
Gene ID 3654
Other Names Interleukin-1 receptor-associated kinase 1, IRAK-1, IRAK1, IRAK
Target/Specificity IRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsIRAK1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )


This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.


Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Molecular mechanism underlying the inflammatory complication of leptin in macrophages. Vaughan T, et al. Mol Immunol, 2010 Sep. PMID 20619458.The role of microRNA-146a (miR-146a) and its target IL-1R-associated kinase (IRAK1) in psoriatic arthritis susceptibility. Chatzikyriakidou A, et al. Scand J Immunol, 2010 May. PMID 20500689.Role of polymorphic variants as genetic modulators of infection in neonatal sepsis. Abu-Maziad A, et al. Pediatr Res, 2010 Oct. PMID 20463618.Association between anti-tumour necrosis factor treatment response and genetic variants within the TLR and NF{kappa}B signalling pathways. Potter C, et al. Ann Rheum Dis, 2010 Jul. PMID 20448286.

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$ 315.00
Cat# AT2538a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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