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IRF4 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant IRF4.

  • WB - IRF4 Antibody (monoclonal) (M02) AT2544a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
  • WB - IRF4 Antibody (monoclonal) (M02) AT2544a
    Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M02), clone 2F2.

    Lane 1: IRF4 transfected lysate(51.8 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - IRF4 Antibody (monoclonal) (M02) AT2544a
    Immunoperoxidase of monoclonal antibody to IRF4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 0.3 ug/ml]
  • E - IRF4 Antibody (monoclonal) (M02) AT2544a
    Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15306
Other Accession NM_002460
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2F2
Calculated MW 51772 Da
Additional Information
Gene ID 3662
Other Names Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, LSIRF, Multiple myeloma oncogene 1, NF-EM5, IRF4, MUM1
Target/Specificity IRF4 (NP_002451, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsIRF4 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene.


Interferon-regulatory factor 4 is essential for the developmental program of T helper 9 cells. Staudt V, et al. Immunity, 2010 Aug 27. PMID 20674401.Melanocytic nevi, nevus genes, and melanoma risk in a large case-control study in the United Kingdom. Newton-Bishop JA, et al. Cancer Epidemiol Biomarkers Prev, 2010 Aug. PMID 20647408.IRF4 variants have age-specific effects on nevus count and predispose to melanoma. Duffy DL, et al. Am J Hum Genet, 2010 Jul 9. PMID 20602913.Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection. Ucisik-Akkaya E, et al. Mol Hum Reprod, 2010 Oct. PMID 20587610.Web-based, participant-driven studies yield novel genetic associations for common traits. Eriksson N, et al. PLoS Genet, 2010 Jun 24. PMID 20585627.

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$ 315.00
Cat# AT2544a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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