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IRF5 Antibody (monoclonal) (M03)
Mouse monoclonal antibody raised against a partial recombinant IRF5.
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Immunofluorescence of monoclonal antibody to IRF5 on A-431 cell. [antibody concentration 10 ug/ml]
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Immunoprecipitation of IRF5 transfected lysate using anti-IRF5 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IRF5 MaxPab mouse polyclonal antibody.
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Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
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IRF5 monoclonal antibody (M03), clone 1H6 Western Blot analysis of IRF5 expression in A-431 ( (Cat # AT2547a )
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IRF5 monoclonal antibody (M03), clone 1H6. Western Blot analysis of IRF5 expression in NIH/3T3((Cat # AT2547a )
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Detection limit for recombinant GST tagged IRF5 is approximately 0.1ng/ml as a capture antibody.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, IP, E |
|---|---|
| Primary Accession | Q13568 |
| Other Accession | NM_002200 |
| Reactivity | Human, Mouse |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 kappa |
| Clone Names | 1H6 |
| Calculated MW | 56044 Da |
| Gene ID | 3663 |
|---|---|
| Other Names | Interferon regulatory factor 5, IRF-5, IRF5 |
| Target/Specificity | IRF5 (NP_002191, 395 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 IP~~N/A E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | IRF5 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.
References
Genome-wide meta-analyses identify three loci associated with primary biliary cirrhosis. Liu X, et al. Nat Genet, 2010 Aug. PMID 20639880.Variants at IRF5-TNPO3, 17q12-21 and MMEL1 are associated with primary biliary cirrhosis. Hirschfield GM, et al. Nat Genet, 2010 Aug. PMID 20639879.Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Genetic Differences between Five European Populations. Moskvina V, et al. Hum Hered, 2010 Jul 8. PMID 20616560.Dengue hemorrhagic fever is associated with polymorphisms in JAK1. Silva LK, et al. Eur J Hum Genet, 2010 Jun 30. PMID 20588308.
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