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KRT20 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant KRT20.
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Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell. [antibody concentration 10 ug/ml]
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Immunofluorescence of monoclonal antibody to KRT20 on U-2 OS cell. [antibody concentration 10 ug/ml]
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Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (72.38 KDa) .
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KRT20 monoclonal antibody (M01), clone 2G3-1C8 Western Blot analysis of KRT20 expression in A-431 ( (Cat # AT2657a )
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Western Blot analysis of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody (M01), clone 2G3-1C8.
Lane 1: KRT20 transfected lysate(48.5 KDa).
Lane 2: Non-transfected lysate.
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Immunoperoxidase of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml]
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Detection limit for recombinant GST tagged KRT20 is approximately 0.03ng/ml as a capture antibody.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | P35900 |
| Other Accession | BC031559 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 kappa |
| Clone Names | 2G3-1C8 |
| Calculated MW | 48487 Da |
| Gene ID | 54474 |
|---|---|
| Other Names | Keratin, type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20 |
| Target/Specificity | KRT20 (AAH31559, 1 a.a. ~ 424 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | KRT20 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
References
The clinical implications of MMP-11 and CK-20 expression in human breast cancer. Cheng CW, et al. Clin Chim Acta, 2010 Feb. PMID 19914229. Identification of 15 loci influencing height in a Korean population. Kim JJ, et al. J Hum Genet, 2010 Jan. PMID 19893584. Aberrant expression of class III beta-tubulin in basal cell carcinoma of the skin. Ishida M, et al. Oncol Rep, 2009 Oct. PMID 19724850. [Association of CK20 expression with the progression, metastasis and prognosis of breast cancer] Wang SM, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2009 Aug. PMID 19664394. Detection of epithelial cells by RT-PCR targeting CEA, CK20, and TEM-8 in colorectal carcinoma patients using OncoQuick density gradient centrifugation system. Lagoudianakis EE, et al. J Surg Res, 2009 Aug. PMID 19645077.
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