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MGC10540 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant MGC10540.

  • WB - MGC10540 Antibody (monoclonal) (M01) AT2857a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (45.1 KDa) .
  • WB - MGC10540 Antibody (monoclonal) (M01) AT2857a
    MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Western Blot analysis of MGC10540 expression in Hela S3 NE ( (Cat # AT2857a )
  • IF - MGC10540 Antibody (monoclonal) (M01) AT2857a
    Immunofluorescence of monoclonal antibody to VPS25 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - MGC10540 Antibody (monoclonal) (M01) AT2857a
    Detection limit for recombinant GST tagged VPS25 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BRG1
Other Accession BC006282
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 2E5-2B9
Calculated MW 20748 Da
Additional Information
Gene ID 84313
Other Names Vacuolar protein-sorting-associated protein 25, hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, VPS25, DERP9, EAP20
Target/Specificity MGC10540 (AAH06282, 1 a.a. ~ 176 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMGC10540 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).


Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Im YJ, et al. Dev Cell, 2009 Aug. PMID 19686684.Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII. Bowers K, et al. J Biol Chem, 2006 Feb 24. PMID 16371348.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.

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$ 315.00
Cat# AT2857a
(40 western blots)
Availability: 7-10 days
Bulk Size
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