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MIF Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant MIF.

  • WB - MIF Antibody (monoclonal) (M01) AT2870a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (38.39 KDa) .
  • WB - MIF Antibody (monoclonal) (M01) AT2870a
    Western Blot analysis of MIF expression in transfected 293T cell line by MIF monoclonal antibody (M01), clone 2A10-4D3.

    Lane 1: MIF transfected lysate(12.5 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - MIF Antibody (monoclonal) (M01) AT2870a
    Immunoperoxidase of monoclonal antibody to MIF on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma tissue. [antibody concentration 1 ~ 10 ug/ml]
  • IP - MIF Antibody (monoclonal) (M01) AT2870a
    Immunoprecipitation of MIF transfected lysate using anti-MIF monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MIF MaxPab rabbit polyclonal antibody.
  • E - MIF Antibody (monoclonal) (M01) AT2870a
    Detection limit for recombinant GST tagged MIF is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14174
Other Accession BC000447
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2A10-4D3
Calculated MW 12476 Da
Additional Information
Gene ID 4282
Other Names Macrophage migration inhibitory factor, MIF, Glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, MIF, GLIF, MMIF
Target/Specificity MIF (AAH00447.1, 1 a.a. ~ 115 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMIF Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.


1.An HLA-Presented Fragment of Macrophage Migration Inhibitory Factor Is a Therapeutic Target for Invasive Breast Cancer.Hawkins O, Verma B, Lightfoot S, Jain R, Rawat A, McNair S, Caseltine S, Mojsilovic A, Gupta P, Neethling F, Almanza O, Dooley W, Hildebrand W, Weidanz J.J Immunol. 2011 Apr 22. [Epub ahead of print]2.eIF3m expression influences the regulation of tumorigenesis-related genes in human colon cancer.Goh SH, Hong SH, Hong SH, Lee BC, Ju MH, Jeong JS, Cho YR, Kim IH, Lee YS.Oncogene. 2010 Sep 13. [Epub ahead of print]

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$ 315.00
Cat# AT2870a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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