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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   NFX1 Antibody (monoclonal) (M01)   

NFX1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NFX1.

  • WB - NFX1 Antibody (monoclonal) (M01) AT3039a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - NFX1 Antibody (monoclonal) (M01) AT3039a
    NFX1 monoclonal antibody (M01), clone 1D12 Western Blot analysis of NFX1 expression in Hela S3 NE ( (Cat # AT3039a )
  • IF - NFX1 Antibody (monoclonal) (M01) AT3039a
    Immunofluorescence of monoclonal antibody to NFX1 on HeLa cell. [antibody concentration 30 ug/ml]
  • E - NFX1 Antibody (monoclonal) (M01) AT3039a
    Detection limit for recombinant GST tagged NFX1 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q12986
Other Accession NM_002504
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1D12
Calculated MW 124395 Da
Additional Information
Gene ID 4799
Other Names Transcriptional repressor NF-X1, 632-, Nuclear transcription factor, X box-binding protein 1, NFX1, NFX2
Target/Specificity NFX1 (NP_002495, 981 a.a. ~ 1080 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNFX1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.


NFX1-123 increases hTERT expression and telomerase activity posttranscriptionally in human papillomavirus type 16 E6 keratinocytes. Katzenellenbogen RA, et al. J Virol, 2009 Jul. PMID 19369336.NFX1 interacts with mSin3A/histone deacetylase to repress hTERT transcription in keratinocytes. Xu M, et al. Mol Cell Biol, 2008 Aug. PMID 18505829.Distinct class of putative non-conserved promoters in humans: comparative studies of alternative promoters of human and mouse genes. Tsuritani K, et al. Genome Res, 2007 Jul. PMID 17567985.NFX1-123 and poly(A) binding proteins synergistically augment activation of telomerase in human papillomavirus type 16 E6-expressing cells. Katzenellenbogen RA, et al. J Virol, 2007 Apr. PMID 17267499.Nrf1 is targeted to the endoplasmic reticulum membrane by an N-terminal transmembrane domain. Inhibition of nuclear translocation and transacting function. Wang W, et al. J Biol Chem, 2006 Jul 14. PMID 16687406.

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$ 315.00
Cat# AT3039a
(40 western blots)
Availability: 7-10 days
Bulk Size
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