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NID1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NID1.

  • WB - NID1 Antibody (monoclonal) (M01) AT3050a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - NID1 Antibody (monoclonal) (M01) AT3050a
    Western Blot analysis of NID1 expression in transfected 293T cell line by NID1 monoclonal antibody (M01), clone 1G3.

    Lane 1: NID1 transfected lysate(122.1 KDa).
    Lane 2: Non-transfected lysate.
  • E - NID1 Antibody (monoclonal) (M01) AT3050a
    Detection limit for recombinant GST tagged NID1 is 1 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14543
Other Accession NM_002508
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1G3
Calculated MW 136377 Da
Additional Information
Gene ID 4811
Other Names Nidogen-1, NID-1, Entactin, NID1, NID
Target/Specificity NID1 (NP_002499.1, 1148 a.a. ~ 1247 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNID1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.


Nidogen 1 and 2 gene promoters are aberrantly methylated in human gastrointestinal cancer. Ulazzi L, et al. Mol Cancer, 2007 Feb 28. PMID 17328794.Lack of nidogen-1 and -2 prevents basement membrane assembly in skin-organotypic coculture. Nischt R, et al. J Invest Dermatol, 2007 Mar. PMID 17008882.The DNA sequence and biological annotation of human chromosome 1. Gregory SG, et al. Nature, 2006 May 18. PMID 16710414.Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo. Lee HK, et al. FEBS Lett, 2006 Apr 17. PMID 16574105.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.

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$ 315.00
Cat# AT3050a
(40 western blots)
Availability: 7-10 days
Bulk Size
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