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NODAL Antibody (monoclonal) (M03)
Mouse monoclonal antibody raised against a partial recombinant NODAL.
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Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]
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Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (33.66 KDa) .
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NODAL monoclonal antibody (M03), clone 5C3 Western Blot analysis of NODAL expression in HeLa ( (Cat # AT3074a )
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NODAL monoclonal antibody (M03), clone 5C3. Western Blot analysis of NODAL expression in PC-12 ( (Cat # AT3074a )
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NODAL monoclonal antibody (M03), clone 5C3. Western Blot analysis of NODAL expression in Raw 264.7 ( (Cat # AT3074a )
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Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]
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Detection limit for recombinant GST tagged NODAL is approximately 0.3ng/ml as a capture antibody.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF |
|---|---|
| Primary Accession | Q96S42 |
| Other Accession | NM_018055 |
| Reactivity | Human, Mouse, Rat |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 5C3 |
| Calculated MW | 39561 Da |
| Gene ID | 4838 |
|---|---|
| Other Names | Nodal homolog, NODAL |
| Target/Specificity | NODAL (NP_060525, 275 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IHC~~1:100~500 IF~~1:50~200 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | NODAL Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development.
References
1.Reactivation of embryonic nodal signaling is associated with tumor progression and promotes the growth of prostate cancer cells.Lawrence MG, Margaryan NV, Loessner D, Collins A, Kerr KM, Turner M, Seftor EA, Stephens CR, Lai J, Postovit LM, Clements JA, Hendrix MJ; APC BioResource.Prostate. 2011 Jan 12. [Epub ahead of print]
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