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NRF1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NRF1.

  • WB - NRF1 Antibody (monoclonal) (M01) AT3114a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.09 KDa) .
  • WB - NRF1 Antibody (monoclonal) (M01) AT3114a
    NRF1 monoclonal antibody (M01), clone 2F9. Western Blot analysis of NRF1 expression in human Skeletal muscle.
  • WB - NRF1 Antibody (monoclonal) (M01) AT3114a
    NRF1 monoclonal antibody (M01), clone 2F9. Western Blot analysis of NRF1 expression in HeLa.
  • WB - NRF1 Antibody (monoclonal) (M01) AT3114a
    Western Blot analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.

    Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - NRF1 Antibody (monoclonal) (M01) AT3114a
    Immunoperoxidase of monoclonal antibody to NRF1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
  • E - NRF1 Antibody (monoclonal) (M01) AT3114a
    Detection limit for recombinant GST tagged NRF1 is approximately 0.03ng/ml as a capture antibody.
  • WB - NRF1 Antibody (monoclonal) (M01) AT3114a
    Western blot analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( (Cat # AT3114a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16656
Other Accession NM_005011
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2F9
Calculated MW 53541 Da
Additional Information
Gene ID 4899
Other Names Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1
Target/Specificity NRF1 (NP_005002, 201 a.a. ~ 285 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNRF1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for nuclear factor (erythroid-derived 2)-like 1 which has an official symbol of NFE2L1.


1.Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex.Thompson C, Macdonald G, Mueller CR.Mol Cancer. 2011 May 24;10(1):62. [Epub ahead of print]

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$ 315.00
Cat# AT3114a
(40 western blots)
Availability: 7-10 days
Bulk Size
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