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NRP1 Antibody (monoclonal) (M05)

Mouse monoclonal antibody raised against a partial recombinant NRP1.

     
  • IP - NRP1 Antibody (monoclonal) (M05) AT3115a
    Immunoprecipitation of NRP1 transfected lysate using anti-NRP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NRP1 MaxPab rabbit polyclonal antibody.
    detail
  • WB - NRP1 Antibody (monoclonal) (M05) AT3115a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (38.21 KDa) .
    detail
  • WB - NRP1 Antibody (monoclonal) (M05) AT3115a
    NRP1 monoclonal antibody (M05), clone 1B3 Western Blot analysis of NRP1 expression in HeLa ( (Cat # AT3115a )
    detail
  • WB - NRP1 Antibody (monoclonal) (M05) AT3115a
    Western Blot analysis of NRP1 expression in transfected 293T cell line by NRP1 monoclonal antibody (M05), clone 1B3.

    Lane 1: NRP1 transfected lysate(68.3 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - NRP1 Antibody (monoclonal) (M05) AT3115a
    Detection limit for recombinant GST tagged NRP1 is approximately 0.03ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP, E
Primary Accession O14786
Other Accession NM_003873
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1B3
Calculated MW 103134 Da
Additional Information
Gene ID 8829
Other Names Neuropilin-1, Vascular endothelial cell growth factor 165 receptor, CD304, NRP1, NRP, VEGF165R
Target/Specificity NRP1 (NP_003864, 22 a.a. ~ 132 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNRP1 Antibody (monoclonal) (M05) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

References

1.Adjuvant effects of formalin-inactivated HSV through activation of dendritic cells and inactivation of myeloid-derived suppressor cells in cancer immunotherapy.Ohkusu-Tsukada K, Ohta S, Kawakami Y, Toda M.Int J Cancer. 2010 Mar 15. [Epub ahead of print]2.EXTRACELLULAR AND MEMBRANE-ASSOCIATED PROSTATE CANCER MARKERSKlee, George G. ;Vasmatzis, George;Kosari, Farhad;Klee, Eric W.FreshPatents.com

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$ 350.00
Cat# AT3115a
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Availability: 7-10 days
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