Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
NUP62 Antibody (monoclonal) (M02)
Mouse monoclonal antibody raised against a partial recombinant NUP62.
-
Immunofluorescence of monoclonal antibody to NUP62 on HeLa cell. [antibody concentration 10 ug/ml]
-
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
-
NUP62 monoclonal antibody (M02), clone 2D3. Western Blot analysis of NUP62 expression in PC-12((Cat # AT3138a )
-
NUP62 monoclonal antibody (M02), clone 2D3 Western Blot analysis of NUP62 expression in Hela S3 NE ( (Cat # AT3138a )
-
NUP62 monoclonal antibody (M02), clone 2D3. Western Blot analysis of NUP62 expression in NIH/3T3 ( (Cat # AT3138a )
-
Western Blot analysis of NUP62 expression in transfected 293T cell line by NUP62 monoclonal antibody (M02), clone 2D3.
Lane 1: NUP62 transfected lysate (Predicted MW: 53.3 KDa).
Lane 2: Non-transfected lysate.
-
Detection limit for recombinant GST tagged NUP62 is approximately 1ng/ml as a capture antibody.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF |
|---|---|
| Primary Accession | P37198 |
| Other Accession | NM_153719 |
| Reactivity | Human, Mouse, Rat |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b Kappa |
| Clone Names | 2D3 |
| Calculated MW | 53255 Da |
| Gene ID | 23636 |
|---|---|
| Other Names | Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62 |
| Target/Specificity | NUP62 (NP_714941, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | NUP62 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.
References
Oxidative stress inhibits nuclear protein export by multiple mechanisms that target FG nucleoporins and Crm1. Crampton N, et al. Mol Biol Cell, 2009 Dec. PMID 19828735.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Mengovirus-induced rearrangement of the nuclear pore complex: hijacking cellular phosphorylation machinery. Bardina MV, et al. J Virol, 2009 Apr. PMID 19144712.Nucleophosmin serves as a rate-limiting nuclear export chaperone for the Mammalian ribosome. Maggi LB Jr, et al. Mol Cell Biol, 2008 Dec. PMID 18809582.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Request Quote Here
Ordering Information
Other Products
Shipping Information
