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OCLN Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant OCLN.

  • WB - OCLN Antibody (monoclonal) (M01) AT3143a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - OCLN Antibody (monoclonal) (M01) AT3143a
    OCLN monoclonal antibody (M01), clone 1G7 Western Blot analysis of OCLN expression in HL-60 ( (Cat # AT3143a )
  • IF - OCLN Antibody (monoclonal) (M01) AT3143a
    Immunofluorescence of monoclonal antibody to OCLN on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - OCLN Antibody (monoclonal) (M01) AT3143a
    Immunoprecipitation of OCLN transfected lysate using anti-OCLN monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with OCLN MaxPab rabbit polyclonal antibody.
  • E - OCLN Antibody (monoclonal) (M01) AT3143a
    Detection limit for recombinant GST tagged OCLN is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16625
Other Accession NM_002538
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1G7
Calculated MW 59144 Da
Additional Information
Gene ID 100506658
Other Names Occludin, OCLN
Target/Specificity OCLN (NP_002529, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsOCLN Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.


Nedd4-2 interacts with occludin to inhibit tight junction formation and enhance paracellular conductance in collecting duct epithelia. Raikwar NS, et al. Am J Physiol Renal Physiol, 2010 Aug. PMID 20504882.Splicing diversity of the human OCLN gene and its biological significance for hepatitis C virus entry. Kohaar I, et al. J Virol, 2010 Jul. PMID 20463075.Tight junction-associated MARVEL proteins marveld3, tricellulin, and occludin have distinct but overlapping functions. Raleigh DR, et al. Mol Biol Cell, 2010 Apr 1. PMID 20164257.The tight junction protein, occludin, regulates the directional migration of epithelial cells. Du D, et al. Dev Cell, 2010 Jan 19. PMID 20152177.Identification of MarvelD3 as a tight junction-associated transmembrane protein of the occludin family. Steed E, et al. BMC Cell Biol, 2009 Dec 22. PMID 20028514.

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$ 315.00
Cat# AT3143a
(40 western blots)
Availability: 7-10 days
Bulk Size
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