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PAK1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant PAK1.

  • WB - PAK1 Antibody (monoclonal) (M02) AT3173a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.53 KDa) .
  • WB - PAK1 Antibody (monoclonal) (M02) AT3173a
    Western Blot analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.

    Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - PAK1 Antibody (monoclonal) (M02) AT3173a
    Immunoperoxidase of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
  • IF - PAK1 Antibody (monoclonal) (M02) AT3173a
    Immunofluorescence of monoclonal antibody to PAK1 on HeLa cell. [antibody concentration 35 ug/ml]
  • IP - PAK1 Antibody (monoclonal) (M02) AT3173a
    Immunoprecipitation of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PAK1 monoclonal antibody.
  • E - PAK1 Antibody (monoclonal) (M02) AT3173a
    Detection limit for recombinant GST tagged PAK1 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13153
Other Accession NM_002576
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 4D1
Calculated MW 60647 Da
Additional Information
Gene ID 5058
Other Names Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK, PAK1
Target/Specificity PAK1 (NP_002567, 191 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPAK1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.


1.Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus.Cook D, Del Rayo Sanchez-Carbente M, Lachance C, Radzioch D, Tremblay S, Khandjian EW, Desgroseillers L, Murai KK.PLoS One. 2011;6(10):e26120. Epub 2011 Oct 11.

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$ 315.00
Cat# AT3173a
(40 western blots)
Availability: 7-10 days
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