PCBP2 Antibody (monoclonal) (M07)
Mouse monoclonal antibody raised against a full length recombinant PCBP2.
|Application ||WB, IHC, IF, E|
|Calculated MW||38580 Da|
|Other Names||Poly(rC)-binding protein 2, Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2, PCBP2|
|Target/Specificity||PCBP2 (AAH01155, 1 a.a. ~ 362 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||PCBP2 Antibody (monoclonal) (M07) is for research use only and not for use in diagnostic or therapeutic procedures.|
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The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
1.hnRNP L and NF90 interact with hepatitis C virus 5' terminal untranslated RNA and promote efficient replication.Li Y, Masaki T, Shimakami T, Lemon SMJ Virol. 2014 Apr 9.2.Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.Fujimura K, Sasaki AT, Anderson P.Nucleic Acids Res. 2012 Jun 20.3.Poly(C)-binding protein 2 Interacts with sequences Required for Viral Replication in HCV 5' UTR and Directs HCV RNA Replication through Circularizing the Viral Genome.Wang L, Jeng KS, Lai MM.J Virol. 2011 Jun 1. [Epub ahead of print]4.Identification of RNA Helicase A (RHA) as a New Host Factor in the Replication Cycle of Foot-and-Mouth Disease Virus.Lawrence P, Rieder E.J Virol. 2009 Nov;83(21):11356-66. Epub 2009 Aug 26.5.Selective localization of PCBP2 to cytoplasmic processing bodies.Fujimura K, Katahira J, Kano F, Yoneda Y, Murata M.Biochim Biophys Acta. 2009 May;1793(5):878-87. Epub 2009 Feb 20.6.Rapid Changes of mRNA-binding Protein Levels following Glucose and 3-Isobutyl-1-methylxanthine Stimulation of Insulinoma INS-1 CellsSus C, Czupalla C, Winter C, Pursche T, Knoch KP, Schroeder M, Hoflack B, Solimena M.Mol Cell Proteomics. 2009 Mar;8(3):393-408. Epub 2008 Oct 14.7.A link between SIN1 (MAPKAP1) and poly(rC) binding protein 2 (PCBP2) in counteracting environmental stress.Ghosh D, Srivastava GP, Xu D, Schulz LC, Roberts RM.Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11673-8. Epub 2008 Aug 7.8.Identification of PCBP2, a facilitator of IRES-mediated translation, as a novel constituent of stress granules and processing bodies.Fujimura K, Kano F, Murata M.RNA. 2008 Mar;14(3):425-31. Epub 2008 Jan 3.
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