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>   home   >   Products   >   Primary Antibodies   >   POLA Antibody (monoclonal) (M01)   

POLA Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant POLA.

  • WB - POLA Antibody (monoclonal) (M01) AT3365a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - POLA Antibody (monoclonal) (M01) AT3365a
    POLA monoclonal antibody (M01), clone 3C11 Western Blot analysis of POLA expression in Hela S3 NE ( (Cat # AT3365a )
  • E - POLA Antibody (monoclonal) (M01) AT3365a
    Detection limit for recombinant GST tagged POLA is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09884
Other Accession NM_016937
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3C11
Calculated MW 165913 Da
Additional Information
Gene ID 5422
Other Names DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, POLA1, POLA
Target/Specificity POLA (NP_058633, 1363 a.a. ~ 1462 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPOLA Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication.


Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Physical interactions between Mcm10, DNA, and DNA polymerase alpha. Warren EM, et al. J Biol Chem, 2009 Sep 4. PMID 19608746.Replication stress activates DNA polymerase alpha-associated Chk1. Taricani L, et al. Cell Cycle, 2009 Feb 1. PMID 19177015.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication. Zhu W, et al. Genes Dev, 2007 Sep 15. PMID 17761813.

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$ 315.00
Cat# AT3365a
(40 western blots)
Availability: 7-10 days
Bulk Size
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