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POLD3 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant POLD3.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q15054 |
| Other Accession | NM_006591 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 3E3 |
| Calculated MW | 51400 Da |
| Gene ID | 10714 |
|---|---|
| Other Names | DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, POLD3, KIAA0039 |
| Target/Specificity | POLD3 (NP_006582, 357 a.a. ~ 466 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | POLD3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1 (MIM 174761), POLD2 (MIM 600815), POLD3, and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).
References
Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk. Michiels S, et al. Carcinogenesis, 2009 May. PMID 19237606.X-ray structure of the complex of regulatory subunits of human DNA polymerase delta. Baranovskiy AG, et al. Cell Cycle, 2008 Oct. PMID 18818516.Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol delta. Baranovskiy AG, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2008 Sep 1. PMID 18765914.Phosphorylation of the C subunit (p66) of human DNA polymerase delta. Lemmens L, et al. Biochem Biophys Res Commun, 2008 Mar 7. PMID 18157942.Dynamics of human replication factors in the elongation phase of DNA replication. Masuda Y, et al. Nucleic Acids Res, 2007. PMID 17932049.
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