POLI Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant POLI.
|Application ||WB, E|
|Calculated MW||83006 Da|
|Other Names||DNA polymerase iota, Eta2, RAD30 homolog B, POLI, RAD30B|
|Target/Specificity||POLI (AAH32662, 616 a.a. ~ 715 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||POLI Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1.Rev1, Rev3, or Rev7 siRNA Abolishes Ultraviolet Light-Induced Translesion Replication in HeLa Cells: A Comprehensive Study Using Alkaline Sucrose Density Gradient Sedimentation.Takezawa J, Ishimi Y, Aiba N, Yamada K.J Nucleic Acids. 2010 Dec 1;2010:750296.2.Evidence that in xeroderma pigmentosum variant cells, which lack DNA polymerase eta, DNA polymerase iota causes the very high frequency and unique spectrum of UV-induced mutations.Wang Y, Woodgate R, McManus TP, Mead S, McCormick JJ, Maher VM.Cancer Res. 2007 Apr 1;67(7):3018-26.
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