POLK Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant POLK.
|Application ||WB, IHC, IF, E|
|Calculated MW||98809 Da|
|Other Names||DNA polymerase kappa, DINB protein, DINP, POLK, DINB1|
|Target/Specificity||POLK (AAH50718, 1 a.a. ~ 472 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||POLK Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).
Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk. Monsees GM, et al. Breast Cancer Res Treat, 2010 May 23. PMID 20496165.Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Critical amino acids in human DNA polymerases eta and kappa involved in erroneous incorporation of oxidized nucleotides. Katafuchi A, et al. Nucleic Acids Res, 2010 Jan. PMID 19939936.Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in Vitro: REV1 inserts dC opposite the lesion, and DNA polymerase kappa potentially catalyzes extension reaction from the 3'-dC terminus. Fukuda H, et al. J Biol Chem, 2009 Sep 18. PMID 19628463.Structural and functional elucidation of the mechanism promoting error-prone synthesis by human DNA polymerase kappa opposite the 7,8-dihydro-8-oxo-2'-deoxyguanosine adduct. Irimia A, et al. J Biol Chem, 2009 Aug 14. PMID 19542228.
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