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PPIA Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant PPIA.

     
  • WB - PPIA Antibody (monoclonal) (M01) AT3400a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (43.89 KDa) .
    detail
  • WB - PPIA Antibody (monoclonal) (M01) AT3400a
    PPIA monoclonal antibody (M01), clone 1F4-1B5 Western Blot analysis of PPIA expression in Jurkat ( (Cat # AT3400a )
    detail
  • WB - PPIA Antibody (monoclonal) (M01) AT3400a
    Western Blot analysis of PPIA expression in transfected 293T cell line by PPIA monoclonal antibody (M01), clone 1F4-1B5.

    Lane 1: PPIA transfected lysate(18 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - PPIA Antibody (monoclonal) (M01) AT3400a
    Detection limit for recombinant GST tagged PPIA is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P62937
Other Accession BC000689
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a kappa
Clone Names 1F4-1B5
Calculated MW 18012 Da
Additional Information
Gene ID 5478
Other Names Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, Peptidyl-prolyl cis-trans isomerase A, N-terminally processed, PPIA, CYPA
Target/Specificity PPIA (AAH00689.1, 1 a.a. ~ 165 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPPIA Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

References

1.Peripheral blood monocyte-expressed ANXA2 geneis involved in pathogenesis of osteoporosis in humans.Deng FY, Lei SF, Zhang Y, Zhang YL, Zheng YP, Zhang LS, Pan R, Wang L, Tian Q, Shen H, Zhao M, Lundberg YW, Liu YZ, Papasian CJ, Deng HW.Mol Cell Proteomics. 2011 Aug 4. [Epub ahead of print]2.Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma.Rho JH, Roehrl MH, Wang JY.J Proteome Res. 2009 Dec;8(12):5610-8.

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$ 350.00
Cat# AT3400a
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