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PPOX Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant PPOX.

  • WB - PPOX Antibody (monoclonal) (M01) AT3407a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - PPOX Antibody (monoclonal) (M01) AT3407a
    Western Blot analysis of PPOX expression in transfected 293T cell line by PPOX monoclonal antibody (M01), clone 2F10.

    Lane 1: PPOX transfected lysate(50.8 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - PPOX Antibody (monoclonal) (M01) AT3407a
    Immunoperoxidase of monoclonal antibody to PPOX on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 3 ug/ml]
  • E - PPOX Antibody (monoclonal) (M01) AT3407a
    Detection limit for recombinant GST tagged PPOX is approximately 0.1ng/ml as a capture antibody.
  • WB - PPOX Antibody (monoclonal) (M01) AT3407a
    Western blot analysis of PPOX over-expressed 293 cell line, cotransfected with PPOX Validated Chimera RNAi ( (Cat # AT3407a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P50336
Other Accession BC002357
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2F10
Calculated MW 50765 Da
Additional Information
Gene ID 5498
Other Names Protoporphyrinogen oxidase, PPO, PPOX
Target/Specificity PPOX (AAH02357, 378 a.a. ~ 477 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPPOX Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.


1.Methotrexate enhances 5-aminolevulinic acid-mediated photodynamic therapy-induced killing of human SCC4 cells by upregulation of coproporphyrinogen oxidase.Yang DF, Lee JW, Chen HM, Huang Z, Hsu YCJ Formos Med Assoc. 2014 Jan 29. pii: S0929-6646(13)00452-X. doi: 10.1016/j.jfma.2013.12.005.2.Posttranslational stability of the heme biosynthetic enzyme ferrochelatase is dependent on iron availability and intact iron-sulfur cluster assembly machinery.Crooks DR, Ghosh MC, Haller RG, Tong WH, Rouault TA.Blood. 2010 Jan 28;115(4):860-9. Epub 2009 Nov 25.

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$ 315.00
Cat# AT3407a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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