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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAPGEF1 Antibody (monoclonal) (M01)   

RAPGEF1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RAPGEF1.

  • WB - RAPGEF1 Antibody (monoclonal) (M01) AT3565a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
  • WB - RAPGEF1 Antibody (monoclonal) (M01) AT3565a
    Western Blot analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 monoclonal antibody (M01), clone 3D10.

    Lane 1: RAPGEF1 transfected lysate(122.7 KDa).
    Lane 2: Non-transfected lysate.
  • E - RAPGEF1 Antibody (monoclonal) (M01) AT3565a
    Detection limit for recombinant GST tagged RAPGEF1 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13905
Other Accession BC041710
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3D10
Calculated MW 120548 Da
Additional Information
Gene ID 2889
Other Names Rap guanine nucleotide exchange factor 1, CRK SH3-binding GNRP, Guanine nucleotide-releasing factor 2, Protein C3G, RAPGEF1, GRF2
Target/Specificity RAPGEF1 (AAH41710, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRAPGEF1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.


1.The WAVE2 complex regulates T cell receptor signaling to integrins via Abl- and CrkL-C3G-mediated activation of Rap1.Nolz JC, Nacusi LP, Segovis CM, Medeiros RB, Mitchell JS, Shimizu Y, Billadeau DD.J Cell Biol. 2008 Sep 22;182(6):1231-44.

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$ 315.00
Cat# AT3565a
(40 western blots)
Availability: 7-10 days
Bulk Size
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