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RASA3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RASA3.

  • WB - RASA3 Antibody (monoclonal) (M01) AT3575a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
  • WB - RASA3 Antibody (monoclonal) (M01) AT3575a
    Western Blot analysis of RASA3 expression in transfected 293T cell line by RASA3 monoclonal antibody (M01), clone 1F11.

    Lane 1: RASA3 transfected lysate (Predicted MW: 95.7 KDa).
    Lane 2: Non-transfected lysate.
  • E - RASA3 Antibody (monoclonal) (M01) AT3575a
    Detection limit for recombinant GST tagged RASA3 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14644
Other Accession BC047242
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a kappa
Clone Names 1F11
Calculated MW 95699 Da
Additional Information
Gene ID 22821
Other Names Ras GTPase-activating protein 3, GAP1(IP4BP), Ins P4-binding protein, RASA3
Target/Specificity RASA3 (AAH47242, 725 a.a. ~ 834 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRASA3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This family member is an inositol 1,3,4,5-tetrakisphosphate-binding protein, like the closely related RAS p21 protein activator 2. The two family members have distinct pleckstrin-homology domains, with this particular member having a domain consistent with its localization to the plasma membrane.


The ability of GAP1IP4BP to function as a Rap1 GTPase-activating protein (GAP) requires its Ras GAP-related domain and an arginine finger rather than an asparagine thumb. Kupzig S, et al. Mol Cell Biol, 2009 Jul. PMID 19433443.Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Rush J, et al. Nat Biotechnol, 2005 Jan. PMID 15592455.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.Analyzing the role of the putative inositol 1,3,4,5-tetrakisphosphate receptor GAP1IP4BP in intracellular Ca2+ homeostasis. Walker SA, et al. J Biol Chem, 2002 Dec 13. PMID 12356770.

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$ 315.00
Cat# AT3575a
(40 western blots)
Availability: 7-10 days
Bulk Size
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