Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
RNASEH1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RNASEH1.
-
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.52 KDa) .
-
RNASEH1 monoclonal antibody (M01), clone 5D10 Western Blot analysis of RNASEH1 expression in HeLa ( (Cat # AT3652a )
-
RNASEH1 monoclonal antibody (M01), clone 5D10. Western Blot analysis of RNASEH1 expression in PC-12 ( (Cat # AT3652a )
-
Western Blot analysis of RNASEH1 expression in transfected 293T cell line by RNASEH1 monoclonal antibody (M01), clone 5D10.
Lane 1: RNASEH1 transfected lysate(32.1 KDa).
Lane 2: Non-transfected lysate.
-
Detection limit for recombinant GST tagged RNASEH1 is approximately 0.03ng/ml as a capture antibody.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O60930 |
| Other Accession | NM_002936 |
| Reactivity | Human, Rat |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 5D10 |
| Calculated MW | 32064 Da |
| Gene ID | 246243 |
|---|---|
| Other Names | Ribonuclease H1, RNase H1, Ribonuclease H type II, RNASEH1, RNH1 |
| Target/Specificity | RNASEH1 (NP_002927, 189 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | RNASEH1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
References
Specific recognition of RNA/DNA hybrid and enhancement of human RNase H1 activity by HBD. Nowotny M, et al. EMBO J, 2008 Apr 9. PMID 18337749.Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription. Nowotny M, et al. Mol Cell, 2007 Oct 26. PMID 17964265.Human RNase H1 discriminates between subtle variations in the structure of the heteroduplex substrate. Lima WF, et al. Mol Pharmacol, 2007 Jan. PMID 17028158.The positional influence of the helical geometry of the heteroduplex substrate on human RNase H1 catalysis. Lima WF, et al. Mol Pharmacol, 2007 Jan. PMID 17028157.Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. Otsuki T, et al. DNA Res, 2005. PMID 16303743.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Request Quote Here
Ordering Information
Other Products
Shipping Information
