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RNF133 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RNF133.
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Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
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Western Blot analysis of RNF133 expression in transfected 293T cell line by RNF133 monoclonal antibody (M01), clone 3D6.
Lane 1: RNF133 transfected lysate(42.3 KDa).
Lane 2: Non-transfected lysate.
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Detection limit for recombinant GST tagged RNF133 is 0.1 ng/ml as a capture antibody.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8WVZ7 |
| Other Accession | NM_139175 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b Kappa |
| Clone Names | 3D6 |
| Calculated MW | 42294 Da |
| Gene ID | 168433 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RNF133, 632-, RING finger protein 133, RNF133 |
| Target/Specificity | RNF133 (NP_631914, 277 a.a. ~ 376 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | RNF133 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene has no intron.
References
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.The DNA sequence of human chromosome 7. Hillier LW, et al. Nature, 2003 Jul 10. PMID 12853948.Human chromosome 7: DNA sequence and biology. Scherer SW, et al. Science, 2003 May 2. PMID 12690205.
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