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RNF20 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RNF20.

  • WB - RNF20 Antibody (monoclonal) (M01) AT3674a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • E - RNF20 Antibody (monoclonal) (M01) AT3674a
    Detection limit for recombinant GST tagged RNF20 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5VTR2
Other Accession NM_019592
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1C6
Calculated MW 113662 Da
Additional Information
Gene ID 56254
Other Names E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, 632-, RING finger protein 20, RNF20, BRE1A
Target/Specificity RNF20 (NP_062538, 177 a.a. ~ 276 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRNF20 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. Yeast BRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and the methylation of histone H3.


Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair. Svendsen JM, et al. Cell, 2009 Jul 10. PMID 19596235.RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells. Kim J, et al. Cell, 2009 May 1. PMID 19410543.Human BRE1 is an E3 ubiquitin ligase for Ebp1 tumor suppressor. Liu Z, et al. Mol Biol Cell, 2009 Feb. PMID 19037095.The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression. Shema E, et al. Genes Dev, 2008 Oct 1. PMID 18832071.Chromatid cohesion defects may underlie chromosome instability in human colorectal cancers. Barber TD, et al. Proc Natl Acad Sci U S A, 2008 Mar 4. PMID 18299561.

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$ 315.00
Cat# AT3674a
(40 western blots)
Availability: 7-10 days
Bulk Size
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