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>   home   >   Products   >   Primary Antibodies   >   SAFB Antibody (monoclonal) (M04)   

SAFB Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a partial recombinant SAFB.

  • WB - SAFB Antibody (monoclonal) (M04) AT3767a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) .
  • WB - SAFB Antibody (monoclonal) (M04) AT3767a
    SAFB monoclonal antibody (M04), clone 5A11 Western Blot analysis of SAFB expression in Hela S3 NE ( (Cat # AT3767a )
  • IHC - SAFB Antibody (monoclonal) (M04) AT3767a
    Immunoperoxidase of monoclonal antibody to SAFB on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
  • IF - SAFB Antibody (monoclonal) (M04) AT3767a
    Immunofluorescence of monoclonal antibody to SAFB on HeLa cell. [antibody concentration 10 ug/ml]
  • E - SAFB Antibody (monoclonal) (M04) AT3767a
    Detection limit for recombinant GST tagged SAFB is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15424
Other Accession NM_002967
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 5A11
Calculated MW 102642 Da
Additional Information
Gene ID 6294
Other Names Scaffold attachment factor B1, SAF-B, SAF-B1, HSP27 estrogen response element-TATA box-binding protein, HSP27 ERE-TATA-binding protein, SAFB, HAP, HET, SAFB1
Target/Specificity SAFB (NP_002958, 111 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSAFB Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
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This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.


SAFB1 mediates repression of immune regulators and apoptotic genes in breast cancer cells. Hammerich-Hille S, et al. J Biol Chem, 2010 Feb 5. PMID 19901029.The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2. Tsianou D, et al. FEBS J, 2009 Sep. PMID 19674106.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Low SAFB levels are associated with worse outcome in breast cancer patients. Hammerich-Hille S, et al. Breast Cancer Res Treat, 2010 Jun. PMID 19137425.Estradiol and tamoxifen mediate rescue of the dominant-negative effects of estrogen response element-binding protein in vivo and in vitro. Chen H, et al. Endocrinology, 2009 May. PMID 19106221.

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$ 315.00
Cat# AT3767a
(40 western blots)
Availability: 7-10 days
Bulk Size
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