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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   SPDEF Antibody (monoclonal) (M01)   

SPDEF Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant SPDEF.

  • WB - SPDEF Antibody (monoclonal) (M01) AT4018a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
  • WB - SPDEF Antibody (monoclonal) (M01) AT4018a
    Western Blot analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5.

    Lane 1: SPDEF transfected lysate(37.518 KDa).
    Lane 2: Non-transfected lysate.
  • E - SPDEF Antibody (monoclonal) (M01) AT4018a
    Detection limit for recombinant GST tagged SPDEF is 0.3 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95238
Other Accession NM_012391
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 4A5
Calculated MW 37518 Da
Additional Information
Gene ID 25803
Other Names SAM pointed domain-containing Ets transcription factor, Prostate epithelium-specific Ets transcription factor, Prostate-specific Ets, Prostate-derived Ets factor, SPDEF, PDEF, PSE
Target/Specificity SPDEF (NP_036523, 92 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSPDEF Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.


Transcriptional regulation of p21/CIP1 cell cycle inhibitor by PDEF controls cell proliferation and mammary tumor progression. Schaefer JS, et al. J Biol Chem, 2010 Apr 9. PMID 20139077.PDEF is a negative regulator of colon cancer cell growth and migration. Moussa O, et al. J Cell Biochem, 2009 Dec 15. PMID 19830706.SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells. Noah TK, et al. Exp Cell Res, 2010 Feb 1. PMID 19786015.Sam-pointed domain containing Ets transcription factor in luminal breast cancer pathogenesis. Sood AK, et al. Cancer Epidemiol Biomarkers Prev, 2009 Jun. PMID 19505923.Proteomic analysis of a PDEF Ets transcription factor-interacting protein complex. Cho JY, et al. J Proteome Res, 2009 Mar. PMID 19203193.

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$ 315.00
Cat# AT4018a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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