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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   SPI1 Antibody (monoclonal) (M02)   

SPI1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant SPI1.

  • WB - SPI1 Antibody (monoclonal) (M02) AT4022a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (38.94 KDa) .
  • WB - SPI1 Antibody (monoclonal) (M02) AT4022a
    SPI1 monoclonal antibody (M02), clone 2G1. Western Blot analysis of SPI1 expression in Raw 264.7.
  • IF - SPI1 Antibody (monoclonal) (M02) AT4022a
    Immunofluorescence of monoclonal antibody to SPI1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - SPI1 Antibody (monoclonal) (M02) AT4022a
    Detection limit for recombinant GST tagged SPI1 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17947
Other Accession NM_003120
Reactivity Human, Mouse
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2G1
Calculated MW 31083 Da
Additional Information
Gene ID 6688
Other Names Transcription factor PU1, 31 kDa-transforming protein, SPI1
Target/Specificity SPI1 (NP_003111, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSPI1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.


PU.1 is regulated by NF-kappaB through a novel binding site in a 17 kb upstream enhancer element. Bonadies N, et al. Oncogene, 2010 Feb 18. PMID 19966852.Heterozygous deletion of the PU.1 locus in human AML. Bonadies N, et al. Blood, 2010 Jan 14. PMID 19890096.PU.1 regulates positive regulatory domain I-binding factor 1/Blimp-1 transcription in lymphoma cells. Desai S, et al. J Immunol, 2009 Nov 1. PMID 19828640.PU.1 activation relieves GATA-1-mediated repression of Cebpa and Cbfb during leukemia differentiation. Burda P, et al. Mol Cancer Res, 2009 Oct. PMID 19825991.PU.1 induces apoptosis in myeloma cells through direct transactivation of TRAIL. Ueno S, et al. Oncogene, 2009 Nov 19. PMID 19749795.

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$ 315.00
Cat# AT4022a
(40 western blots)
Availability: 7-10 days
Bulk Size
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