Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
SPINK1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant SPINK1.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IP, E |
|---|---|
| Primary Accession | P00995 |
| Other Accession | BC025790 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 4D4 |
| Calculated MW | 8507 Da |
| Gene ID | 6690 |
|---|---|
| Other Names | Pancreatic secretory trypsin inhibitor, Serine protease inhibitor Kazal-type 1, Tumor-associated trypsin inhibitor, TATI, SPINK1, PSTI |
| Target/Specificity | SPINK1 (AAH25790, 24 a.a. ~ 79 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | SPINK1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.
References
1.HOXB13 G84E-related Familial Prostate Cancers: A Clinical, Histologic, and Molecular Survey.Smith SC, Palanisamy N, Zuhlke KA, Johnson AM, Siddiqui J, Chinnaiyan AM, Kunju LP, Cooney KA, Tomlins SAAm J Surg Pathol. 2014 May;38(5):615-26. doi: 10.1097/PAS.0000000000000090.2.Novel dual-color immunohistochemical methods for detecting ERG-PTEN and ERG-SPINK1 status in prostate carcinoma.Bhalla R, Kunju LP, Tomlins SA, Christopherson K, Cortez C, Carskadon S, Siddiqui J, Park K, Miguel Mosquera J, Pestano GA, Rubin MA, Chinnaiyan AM, Palanisamy NMod Pathol. 2013 Jan 25. doi: 10.1038/modpathol.2012.234.3.Loss of SPINK1 expression is associated with unfavorable outcomes in urothelial carcinoma of the bladder after radical cystectomy.Rink M, Park K, Volkmer BG, Xylinas E, Hansen J, Cha EK, Robinson BD, Hautmann R, Kufer R, Engel O, Chun FK, Dahlem R, Rubin MA, Shariat SF, Mosquera JM.Urol Oncol. 2012 Sep 1. pii: S1078-1439(12)00237-2. doi: 10.1016/j.urolonc.2012.06.011.4.325 DIFFERENCES IN TMPRSS2-ERG GENE FUSION AND SPINK1 OVEREXPRESSION IN PROSTATE CANCER IN AFRICAN-AMERICAN AND CAUCASIAN MEN.Khani F, Mosquera JM, Park K, Srivastava A, Tewari A, Rubin M, Robinson B.The Journal of Urology Volume 187, Issue 4, Supplement, April 2012, Pages e1325.Characterization of ETS gene aberrations in select histologic variants of prostate carcinoma.Han B, Mehra R, Suleman K, Tomlins SA, Wang L, Singhal N, Linetzky KA, Palanisamy N, Zhou M, Chinnaiyan AM, Shah RB.Mod Pathol. 2009 Sep;22(9):1176-85. Epub 2009 May 22.6.The role of SPINK1 in ETS rearrangement-negative prostate cancers.Tomlins SA, Rhodes DR, Yu J, Varambally S, Mehra R, Perner S, Demichelis F, Helgeson BE, Laxman B, Morris DS, Cao Q, Cao X, Andren O, Fall K, Johnson L, Wei JT, Shah RB, Al-Ahmadie H, Eastham JA, Eggener SE, Fine SW, Hotakainen K, Stenman UH, Tsodikov A,Cancer Cell. 2008 Jun;13(6):519-28.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
