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>   home   >   Products   >   Primary Antibodies   >   TCF12 Antibody (monoclonal) (M01)   

TCF12 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant TCF12.

  • WB - TCF12 Antibody (monoclonal) (M01) AT4182a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) .
  • WB - TCF12 Antibody (monoclonal) (M01) AT4182a
    TCF12 monoclonal antibody (M01), clone 2E9 Western Blot analysis of TCF12 expression in Jurkat ( (Cat # AT4182a )
  • WB - TCF12 Antibody (monoclonal) (M01) AT4182a
    Western Blot analysis of TCF12 expression in transfected 293T cell line by TCF12 monoclonal antibody (M01), clone 2E9.

    Lane 1: TCF12 transfected lysate(75.8 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - TCF12 Antibody (monoclonal) (M01) AT4182a
    Immunoperoxidase of monoclonal antibody to TCF12 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 ug/ml]
  • WB - TCF12 Antibody (monoclonal) (M01) AT4182a
    Western blot analysis of TCF12 over-expressed 293 cell line, cotransfected with TCF12 Validated Chimera RNAi ( (Cat # AT4182a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99081
Other Accession NM_207036
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2E10
Calculated MW 72965 Da
Additional Information
Gene ID 6938
Other Names Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, bHLHb20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4, TCF12, BHLHB20, HEB, HTF4
Target/Specificity TCF12 (NP_996919, 364 a.a. ~ 453 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTCF12 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.


Synergy between IL-15 and Id2 promotes the expansion of human NK progenitor cells, which can be counteracted by the E protein HEB required to drive T cell development. Schotte R, et al. J Immunol, 2010 Jun 15. PMID 20483740.Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors. Landa I, et al. PLoS Genet, 2009 Sep. PMID 19730683.AML1/ETO oncoprotein is directed to AML1 binding regions and co-localizes with AML1 and HEB on its targets. Gardini A, et al. PLoS Genet, 2008 Nov. PMID 19043539.CBFA2T3-ZNF652 corepressor complex regulates transcription of the E-box gene HEB. Kumar R, et al. J Biol Chem, 2008 Jul 4. PMID 18456661.

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$ 315.00
Cat# AT4182a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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