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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRIM25 Antibody (monoclonal) (M02)   

TRIM25 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant TRIM25.

  • WB - TRIM25 Antibody (monoclonal) (M02) AT4346a
    TRIM25 monoclonal antibody (M02), clone 5F12 Western Blot analysis of TRIM25 expression in Hela S3 NE ( Cat # L013V3 ).
  • IF - TRIM25 Antibody (monoclonal) (M02) AT4346a
    Immunofluorescence of monoclonal antibody to TRIM25 on HeLa cell. [antibody concentration 10 ug/ml]
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14258
Other Accession NM_005082
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 5F12
Calculated MW 70973 Da
Additional Information
Gene ID 7706
Other Names E3 ubiquitin/ISG15 ligase TRIM25, 632n3, Estrogen-responsive finger protein, RING finger protein 147, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147, TRIM25, EFP, RNF147, ZNF147
Target/Specificity TRIM25 (NP_005073, 211 a.a. ~ 310 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTRIM25 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.


The role of height-associated loci identified in genome wide association studies in the determination of pediatric stature. Zhao J, et al. BMC Med Genet, 2010 Jun 14. PMID 20546612.Expression of Efp, VEGF and bFGF in normal, hyperplastic and malignant endometrial tissue. Dai H, et al. Oncol Rep, 2010 Mar. PMID 20127022.Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by the host viral RNA sensor RIG-I. Gack MU, et al. Cell Host Microbe, 2009 May 8. PMID 19454348.Influenza A virus TRIMs the type I interferon response. Ludwig S, et al. Cell Host Microbe, 2009 May 8. PMID 19454344.Regulation of the vascular endothelial growth factor and growth by estrogen and antiestrogens through Efp in Ishikawa endometrial carcinoma cells. Dai H, et al. Oncol Rep, 2009 Feb. PMID 19148513.

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$ 315.00
Cat# AT4346a
(40 western blots)
Availability: 7-10 days
Bulk Size
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