TRIM28 Antibody (monoclonal) (M02)
Mouse monoclonal antibody raised against a partial recombinant TRIM28.
|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||88550 Da|
|Other Names||Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, 632-, KRAB-associated protein 1, KAP-1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, Tripartite motif-containing protein 28, TRIM28, KAP1, RNF96, TIF1B|
|Target/Specificity||TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||TRIM28 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.|
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The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
SUMOylation of the transcriptional co-repressor KAP1 is regulated by the serine and threonine phosphatase PP1. Li X, et al. Sci Signal, 2010 Apr 27. PMID 20424263.KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading. Groner AC, et al. PLoS Genet, 2010 Mar 5. PMID 20221260.Aire's partners in the molecular control of immunological tolerance. Abramson J, et al. Cell, 2010 Jan 8. PMID 20085707.53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair. Noon AT, et al. Nat Cell Biol, 2010 Feb. PMID 20081839.Histone deacetylase 10 relieves repression on the melanogenic program by maintaining the deacetylation status of repressors. Lai IL, et al. J Biol Chem, 2010 Mar 5. PMID 20032463.
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