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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   TRIM28 Antibody (monoclonal) (M02)   

TRIM28 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant TRIM28.

  • WB - TRIM28 Antibody (monoclonal) (M02) AT4349a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (41.69 KDa) .
  • WB - TRIM28 Antibody (monoclonal) (M02) AT4349a
    TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in PC-12 ( (Cat # AT4349a )
  • WB - TRIM28 Antibody (monoclonal) (M02) AT4349a
    TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in MCF-7.
  • WB - TRIM28 Antibody (monoclonal) (M02) AT4349a
    TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in NIH/3T3 ( (Cat # AT4349a )
  • WB - TRIM28 Antibody (monoclonal) (M02) AT4349a
    Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M02), clone 1D11.

    Lane 1: TRIM28 transfected lysate (Predicted MW: 88.5 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - TRIM28 Antibody (monoclonal) (M02) AT4349a
    Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13263
Other Accession BC004978
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1D11
Calculated MW 88550 Da
Additional Information
Gene ID 10155
Other Names Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, 632-, KRAB-associated protein 1, KAP-1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, Tripartite motif-containing protein 28, TRIM28, KAP1, RNF96, TIF1B
Target/Specificity TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTRIM28 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.


SUMOylation of the transcriptional co-repressor KAP1 is regulated by the serine and threonine phosphatase PP1. Li X, et al. Sci Signal, 2010 Apr 27. PMID 20424263.KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading. Groner AC, et al. PLoS Genet, 2010 Mar 5. PMID 20221260.Aire's partners in the molecular control of immunological tolerance. Abramson J, et al. Cell, 2010 Jan 8. PMID 20085707.53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair. Noon AT, et al. Nat Cell Biol, 2010 Feb. PMID 20081839.Histone deacetylase 10 relieves repression on the melanogenic program by maintaining the deacetylation status of repressors. Lai IL, et al. J Biol Chem, 2010 Mar 5. PMID 20032463.

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$ 315.00
Cat# AT4349a
(40 western blots)
Availability: 7-10 days
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