USP14 Antibody (monoclonal) (M08)
Mouse monoclonal antibody raised against a partial recombinant USP14.
|Application ||WB, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||56069 Da|
|Other Names||Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14, USP14, TGT|
|Target/Specificity||USP14 (NP_005142, 395 a.a. ~ 494 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||USP14 Antibody (monoclonal) (M08) is for research use only and not for use in diagnostic or therapeutic procedures.|
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This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions. Chen PC, et al. J Neurosci, 2009 Sep 2. PMID 19726649.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.USP14 inhibits ER-associated degradation via interaction with IRE1alpha. Nagai A, et al. Biochem Biophys Res Commun, 2009 Feb 20. PMID 19135427.Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK ativation. Mines MA, et al. J Biol Chem, 2009 Feb 27. PMID 19106094.
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