|Application ||WB, IHC-P, IF|
|Calculated MW||H=22 KDa|
|Antigen Region||1-217 aa|
|Other Names||MGMT;Methylated-DNA--protein-cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA-alkyltransferase|
|Target/Specificity||Purified His-tagged MGMT protein was used to produced this monoclonal antibody.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.|
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Provided below are standard protocols that you may find useful for product applications.
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Edara S., et al. Carcinogenesis 16:1637-1642(1995).
Tano K., et al. Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990).
Rydberg B., et al. J. Biol. Chem. 265:9563-9569(1990).
Koike G., et al. J. Biol. Chem. 265:14754-14762(1990).
Hayakawa H., et al. J. Mol. Biol. 213:739-747(1990).
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