|Application ||IHC-P, WB|
|Calculated MW||H=17;M=17;Rat=17 KDa|
|Antigen Region||1-132 aa|
|Other Names||NME1;NDPKA; NM23; Nucleoside diphosphate kinase A; Nucleoside diphosphate kinase A; Granzyme A-activated DNase; Nucleoside diphosphate kinase A; Metastasis inhibition factor nm23; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein; Nucleoside diphosphate kinase A; nm23-H1|
|Target/Specificity||Purified His-tagged NME1 protein was used to produced this monoclonal antibody.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NME1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.|
|Cellular Location||Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA|
|Tissue Location||Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.|
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Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.
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Gilles A.-M., et al. J. Biol. Chem. 266:8784-8789(1991).
Wang L., et al. Cancer Res. 53:717-720(1993).
Dooley S., et al. Hum. Genet. 93:63-66(1994).
Ni X., et al. J. Hum. Genet. 48:96-100(2003).
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