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HSP90B1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - HSP90B1 Antibody (Center) AW5268-U400
    Western blot analysis of lysates from mouse C2C12,MCF-7,Hela cell line (from left to right), using HSP90B1 Antibody (Center)(Cat. #AW5268). AW5268 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • IHC-P - HSP90B1 Antibody (Center) AW5268-U400
    HSP90B1 Antibody (Center) (Cat. #AW5268)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSP90B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - HSP90B1 Antibody (Center) AW5268-U400
    HSP90B1 Antibody (Center) (Cat. #AW5268) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14625
Other Accession Q4R520, NP_003290.1
Reactivity Human, Rat
Predicted Mouse, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW H=92;M=92;Rat=93,74 KDa
Additional Information
Gene ID 7184
Antigen Region 460-487 aa
Other Names HSP90B1; GRP94; TRA1; Endoplasmin; 94 kDa glucose-regulated protein; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog
Dilution WB~~ 1:1000
Target/Specificity This HSP90B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-487 amino acids from the Central region of human HSP90B1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSP90B1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSP90B1
Synonyms GRP94, TRA1
Function Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Citations (0)

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HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Suzuki, S., et al. Biochem. Biophys. Res. Commun. 398(3):525-531(2010)
Bloor, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6970-6975(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Wang, X., et al. Clin. Dev. Immunol. 2010, 212537 (2010) :

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$ 325.00
$ 110.00
Cat# AW5268-U400
(40 western blots)
Availability: In Stock
Bulk Size
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