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NDUA4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, IF |
|---|---|
| Primary Accession | O00483 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | H=9;M=9 KDa |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 4697 |
|---|---|
| Antigen Region | 56-80 aa |
| Other Names | NDUFA4;NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 |
| Dilution | IF~~1:25 WB~~1:1000 IHC-P~~1:25 |
| Target/Specificity | This NDUA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-80 amino acids from the C-terminal region of human NDUA4. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NDUA4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NDUFA4 |
|---|---|
| Function | Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (PubMed:22902835). NDUFA4 is required for complex IV maintenance (PubMed:22902835). |
| Cellular Location | Mitochondrion inner membrane; Single-pass membrane protein |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
References
Kim J.W., et al. Biochem. Mol. Biol. Int. 43:669-675(1997).
Kanagarajah D., et al. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Ebert L., et al. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Halleck A., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W., et al. Science 300:767-772(2003).
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