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CHML Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - CHML Antibody (C-term) AW5373-U400
    All lanes : Anti-CHML Antibody (C-term) at 1/1000 dilution Lane 1: HT-1080 whole cell lysates Lane 2: Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • FC - CHML Antibody (C-term) AW5373-U400
    Flow cytometric analysis of Hela cells using CHML Antibody (C-term)(green, Cat#AW5373) compared to an isotype control of rabbit IgG(blue). AW5373 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • IHC-P - CHML Antibody (C-term) AW5373-U400
    Immunohistochemical analysis of paraffin-embedded H. stomach section using CHML Antibody (C-term)(Cat#AW5373). AW5373 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - CHML Antibody (C-term) AW5373-U400
    Immunohistochemical analysis of paraffin-embedded R. cerebellum section using CHML Antibody (C-term)(Cat#AW5373). AW5373 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - CHML Antibody (C-term) AW5373-U400
    Immunohistochemical analysis of paraffin-embedded M. cerebellum section using CHML Antibody (C-term)(Cat#AW5373). AW5373 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P
Primary Accession P26374
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=74 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 1122
Antigen Region 624-656 aa
Other Names Rab proteins geranylgeranyltransferase component A 2, Choroideremia-like protein, Rab escort protein 2, REP-2, CHML, REP2
Dilution WB~~1:1000
FC~~1:25
IHC-P~~1:25
Target/Specificity This CHML antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 624-656 amino acids from the C-terminal region of human CHML.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHML Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHML
Synonyms REP2
Function Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
Cellular Location Cytoplasm, cytosol
Citations (0)

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Background

Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.

References

Cremers F.P.M.,et al.Hum. Mol. Genet. 1:71-75(1992).
Kasper G.,et al.Gene 295:27-32(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 325.00
$ 110.00
Cat# AW5373-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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