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EHD2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - EHD2 Antibody (C-term) AW5422-U400
    All lanes : Anti-EHD2 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: human placenta lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - EHD2 Antibody (C-term) AW5422-U400
    Immunohistochemical analysis of paraffin-embedded H. placenta section using EHD2 Antibody (C-term)(Cat# AW5422). AW5422 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IHC-P - EHD2 Antibody (C-term) AW5422-U400
    Immunohistochemical analysis of paraffin-embedded H. heart section using EHD2 Antibody (C-term)(Cat# AW5422). AW5422 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB
Primary Accession Q9NZN4
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=61,46;M=61;R=61 KDa
Additional Information
Gene ID 30846
Antigen Region 415-449 aa
Other Names EH domain-containing protein 2, PAST homolog 2, EHD2, PAST2
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This EHD2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 415-449 amino acids from the C-terminal region of human EHD2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEHD2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EHD2
Synonyms PAST2
Function Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Required for translocation of FER1L5 to the plasma membrane. Binds ATP; does not bind GTP (By similarity).
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein Note=Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes (By similarity).
Tissue Location Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle
Citations (0)

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Background

Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Required for translocation of FER1L5 to the plasma membrane. Binds ATP; does not bind GTP (By similarity).

References

Pohl U.,et al.Genomics 63:255-262(2000).
Benjamin S.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Aboulaich N.,et al.Biochem. J. 383:237-248(2004).

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$ 325.00
$ 110.00
Cat# AW5422-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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