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TPI1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TPI1 Antibody (C-term) AW5440-U400
    All lanes : Anti-TPI1 Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: LNCaP whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - TPI1 Antibody (C-term) AW5440-U400
    Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - TPI1 Antibody (C-term) AW5440-U400
    TPI1 Antibody (C-term) (Cat. #AW5440) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB
Primary Accession P60174
Other Accession P48500, P00939, P17751, Q60HC9, Q5E956
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=31,27,18;M=32;R=27 KDa
Additional Information
Gene ID 7167
Antigen Region 258-286 aa
Other Names Triosephosphate isomerase, TIM, Triose-phosphate isomerase, TPI1, TPI
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Target/Specificity This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the C-terminal region of human TPI1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTPI1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TPI1
Synonyms TPI
Citations (0)

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Background

TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.

References

Martins-de-Souza,D., et.al., BMC Psychiatry 9, 17 (2009)

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$ 325.00
$ 110.00
Cat# AW5440-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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