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PSMB9 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC - PSMB9 Antibody (C-term) AW5487-U400
    AW5487 staining PSMB9 in human spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • FC - PSMB9 Antibody (C-term) AW5487-U400
    Overlay histogram showing Hela cells stained with AW5486 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5487, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - PSMB9 Antibody (C-term) AW5487-U400
    All lanes : Anti-PSMB9 Antibody (C-term) at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P28065
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=23,22;M=23;R=23 KDa
Additional Information
Gene ID 5698
Antigen Region 205-239 aa
Other Names Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein, PSMB9, LMP2, PSMB6i, RING12
Dilution IHC~~1:25
FC~~1:25
WB~~1:1000
Target/Specificity This PSMB9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 205-239 amino acids from the C-terminal region of human PSMB9.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMB9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMB9
Synonyms LMP2, PSMB6i, RING12
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.
Cellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809}. Nucleus
Citations (0)

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Background

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.

References

Glynne R.,et al.Eur. J. Immunol. 23:860-866(1993).
Beck S.,et al.J. Mol. Biol. 228:433-441(1992).
Kelly A.,et al.Nature 353:667-668(1991).
Fruh K.,et al.J. Biol. Chem. 267:22131-22140(1992).
Beck S.,et al.J. Mol. Biol. 255:1-13(1996).

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$ 325.00
$ 110.00
Cat# AW5487-U400
Size:
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(40 western blots)
Availability: In Stock
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