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(Mouse) Epcam Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - (Mouse) Epcam Antibody (C-term) AW5495-U400
    Flow cytometric analysis of HepG2 cells using (Mouse) Epcam Antibody (C-term)(green, Cat#AW5495) compared to an isotype control of rabbit IgG(blue). AW5493 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - (Mouse) Epcam Antibody (C-term) AW5495-U400
    All lanes : Anti-Epcam Antibody (C-term) at 1:1000 dilution Lane 1: mouse kidney lysates Lane 2: mouse lung lysates Lane 3: mouse testis lysates Lane 4: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q99JW5
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW M=35;H=35;R=35 KDa
Additional Information
Gene ID 17075
Antigen Region 302-335 aa
Other Names Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1
Dilution FC~~1:25
WB~~1:1000
Target/Specificity This Mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Epcam Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Epcam
Synonyms Tacstd1
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein. Lateral cell membrane. Cell junction, tight junction Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction.
Citations (0)

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Background

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

References

Bergsagel P.L.,et al.J. Immunol. 148:590-596(1992).
Carninci P.,et al.Science 309:1559-1563(2005).

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$ 325.00
$ 110.00
Cat# AW5495-U400
Size:
Quantity:
(40 western blots)
Availability: In Stock
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