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HNRNPUL2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC - HNRNPUL2 Antibody (Center) AW5598-U200
    AW5598 staining HNRNPUL2 in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - HNRNPUL2 Antibody (Center) AW5598-U200
    All lanes : Anti-HNRNPUL2 Antibody (Center) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q1KMD3
Other Accession Q00PI9
Reactivity Human
Predicted Dog
Host Rabbit
Clonality Polyclonal
Calculated MW H=85;M=85 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Antigen Region 317-350 aa
Other Names Heterogeneous nuclear ribonucleoprotein U-like protein 2, Scaffold-attachment factor A2, SAF-A2, HNRNPUL2, HNRPUL2
Dilution IHC~~1:25
WB~~1:2000
Target/Specificity This HNRNPUL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 317-350 amino acids from the Central region of human HNRNPUL2.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHNRNPUL2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HNRNPUL2
Synonyms HNRPUL2
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q00PI9}. EMBL; DQ470474; ABF00125.1; -; mRNA EMBL; AL834470; CAD39129.2; -; mRNA CCDS; CCDS41659.1; - RefSeq; NP_001073027.1; NM_001079559.2 UniGene; Hs.657058; - ProteinModelPortal; Q1KMD3; - SMR; Q1KMD3; - BioGrid; 128685; 68 IntAct; Q1KMD3; 25 MINT; Q1KMD3; - STRING; 9606.ENSP00000301785; - iPTMnet; Q1KMD3; - PhosphoSitePlus; Q1KMD3; - SwissPalm; Q1KMD3; - BioMuta; HNRNPUL2; - DMDM; 121940728; - EPD; Q1KMD3; - MaxQB; Q1KMD3; - PaxDb; Q1KMD3; - PeptideAtlas; Q1KMD3; - PRIDE; Q1KMD3; - ProteomicsDB; 61219; - DNASU; 221092; - Ensembl; ENST00000301785; ENSP00000301785; ENSG00000214753 GeneID; 221092; - KEGG; hsa:221092; - UCSC; uc001nuw.4; human CTD; 221092; - EuPathDB; HostDB:ENSG00000214753.2; - GeneCards; HNRNPUL2; - HGNC; HGNC:25451; HNRNPUL2 HPA; HPA041632; - HPA; HPA056696; - HPA; HPA067699; - neXtProt; NX_Q1KMD3; - OpenTargets; ENSG00000214753; - PharmGKB; PA162391554; - eggNOG; ENOG410IR1X; Eukaryota eggNOG; ENOG41101YN; LUCA GeneTree; ENSGT00390000020210; - HOGENOM; HOG000253920; - HOVERGEN; HBG061101; - InParanoid; Q1KMD3; - OMA; ACKAEPR; - OrthoDB; EOG091G041T; - PhylomeDB; Q1KMD3; - TreeFam; TF317301; - ChiTaRS; HNRNPUL2; human GenomeRNAi; 221092; - PMAP-CutDB; Q1KMD3; - PRO; PR:Q1KMD3; - Proteomes; UP000005640; Chromosome 11 Bgee; ENSG00000214753; - CleanEx; HS_HNRNPUL2; - Genevisible; Q1KMD3; HS GO; GO:0016020; C:membrane; HDA:UniProtKB GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005634; C:nucleus; ISS:UniProtKB GO; GO:0003723; F:RNA binding; HDA:UniProtKB CDD; cd12884; SPRY_hnRNP; 1 Gene3D; 1.10.720.30; -; 1 InterPro; IPR001870; B30.2/SPRY InterPro; IPR013320; ConA-like_dom_sf InterPro; IPR027417; P-loop_NTPase InterPro; IPR003034; SAP_dom InterPro; IPR036361; SAP_dom_sf InterPro; IPR003877; SPRY_dom InterPro; IPR035778; SPRY_hnRNP_U Pfam; PF02037; SAP; 1 Pfam; PF00622; SPRY; 1 SMART; SM00513; SAP; 1 SMART; SM00449; SPRY; 1 SUPFAM; SSF49899; SSF49899; 1 SUPFAM; SSF52540; SSF52540; 1 SUPFAM; SSF68906; SSF68906; 1 PROSITE; PS50188; B302_SPRY; 1 PROSITE; PS50800; SAP; 1 1: Evidence at protein level; Complete proteome; Direct protein sequencing; Methylation; Nucleus; Phosphoprotein; Reference proteome CHAIN 1 747 Heterogeneous nuclear ribonucleoprotein U-like protein 2 /FTId=PRO_0000278142 DOMAIN 3 37 SAP. {ECO:0000255|PROSITE- ProRule:PRU00186} DOMAIN 226 419 B30.2/SPRY. {ECO:0000255|PROSITE- ProRule:PRU00548} COMPBIAS 75 241 Glu-rich. COMPBIAS 668 746 Tyr-rich. MOD_RES 161 161 Phosphoserine {ECO:0000250|UniProtKB:Q00PI9} MOD_RES 165 165 Phosphothreonine {ECO:0000250|UniProtKB:Q00PI9} MOD_RES 168 168 Phosphoserine {ECO:0000250|UniProtKB:Q00PI9} MOD_RES 185 185 Phosphoserine {ECO:0000250|UniProtKB:Q00PI9} MOD_RES 188 188 Phosphoserine {ECO:0000250|UniProtKB:Q00PI9} MOD_RES 226 226 Phosphoserine {ECO:0000250|UniProtKB:Q00PI9} MOD_RES 228 228 Phosphoserine. {ECO:0000269|Ref.3} MOD_RES 656 656 Omega-N-methylarginine {ECO:0000250|UniProtKB:Q00PI9} MOD_RES 684 684 Omega-N-methylarginine {ECO:0000250|UniProtKB:Q00PI9} MOD_RES 738 738 Omega-N-methylarginine MOD_RES 747 747 Omega-N-methylarginine SEQUENCE 747 AA; 85105 MW; 7EAAC2F598330AAA CRC64; MEVKRLKVTE LRSELQRRGL DSRGLKVDLA QRLQEALDAE MLEDEAGGGG AGPGGACKAE PRPVAASGGG PGGDEEEDEE EEEEDEEALL EDEDEEPPPA QALGQAAQPP PEPPEAAAME AAAEPDASEK PAEATAGSGG VNGGEEQGLG KREEDEPEER SGDETPGSEV PGDKAAEEQG DDQDSEKSKP AGSDGERRGV KRQRDEKDEH GRAYYEFREE AYHSRSKSPL PPEEEAKDEE EDQTLVNLDT YTSDLHFQVS KDRYGGQPLF SEKFPTLWSG ARSTYGVTKG KVCFEAKVTQ NLPMKEGCTE VSLLRVGWSV DFSRPQLGED EFSYGFDGRG LKAENGQFEE FGQTFGENDV IGCFANFETE EVELSFSKNG EDLGVAFWIS KDSLADRALL PHVLCKNCVV ELNFGQKEEP FFPPPEEFVF IHAVPVEERV RTAVPPKTIE ECEVILMVGL PGSGKTQWAL KYAKENPEKR YNVLGAETVL NQMRMKGLEE PEMDPKSRDL LVQQASQCLS KLVQIASRTK RNFILDQCNV YNSGQRRKLL LFKTFSRKVV VVVPNEEDWK KRLELRKEVE GDDVPESIML EMKANFSLPE KCDYMDEVTY GELEKEEAQP IVTKYKEEAR KLLPPSEKRT NRRNNRNKRN RQNRSRGQGY VGGQRRGYDN RAYGQQYWGQ PGNRGGYRNF YDRYRGDYDR FYGRDYEYNR YRDYYRQYNR DWQSYYYHHP QDRDRYYRNY YGYQGYR
Research Areas
Citations (0)

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References

Herrmann F.,et al.Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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$ 325.00
$ 110.00
Cat# AW5598-U200
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(40 western blots)
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