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DFFA Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DFFA Antibody (C-term) AW5601-U100
    All lanes : Anti-DFFA Antibody (C-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: A431 whole cell lysate Lane 5: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O00273
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW H=37 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 1676
Antigen Region 304-331 aa
Other Names DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, DFF45
Dilution WB~~1:2000
Target/Specificity This DFFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-331 amino acids from the C-terminal region of human DFFA.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDFFA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DFFA
Synonyms DFF1, DFF45
Function Inhibitor of the caspase-activated DNase (DFF40).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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Background

DFFA is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

References

Ninios,Y.P., et.al., Apoptosis 15 (2), 128-138 (2010)
Banas,T., et.al., Eur. J. Obstet. Gynecol. Reprod. Biol. 146 (1), 87-91 (2009)
Trynka,G., et.al., Gut 58 (8), 1078-1083 (2009)

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$ 370.00
Cat# AW5601-U100
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