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ENASE Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - ENASE Antibody  (Center) AW5630-U200
    All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: mouse testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - ENASE Antibody  (Center) AW5630-U200
    ENASE Antibody (Center) (Cat. #AW5630) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ENASE Antibody  (Center) AW5630-U200
    ENASE Antibody (Center) (Cat. #AW5630) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NFI3
Other Accession Q8BX80
Reactivity Human, Mouse
Predicted Dog
Host Rabbit
Clonality Polyclonal
Calculated MW H=84,43;M=83 KDa
Additional Information
Gene ID 64772
Antigen Region 326-354 aa
Other Names Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE
Dilution WB~~0.25
Target/Specificity This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENASE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
Cellular Location Cytoplasm, cytosol.
Tissue Location Widely expressed. Expressed at higher level in thymus and spleen.
Research Areas
Citations (0)

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Endo-beta-N-acetylglucosaminidase (ENGase; EC is involved in the processing of free oligosaccharides in the cytosol.


Park, K., et al. Diabetes 59(7):1845-1850(2010)
Dorfmueller, H.C., et al. FEBS Lett. 584(4):694-700(2010)
Chantret, I., et al. PLoS ONE 5 (7), E11734 (2010) :
Butkinaree, C., et al. J. Biol. Chem. 283(35):23557-23566(2008)
Ortutay, Z., et al. Arthritis Rheum. 48(8):2163-2172(2003)

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$ 325.00
$ 110.00
Cat# AW5630-U200
(40 western blots)
Availability: In Stock
Bulk Size
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