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EEF1E1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • IF - EEF1E1 Antibody AW5638-U100
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AW5638 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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  • WB - EEF1E1 Antibody AW5638-U100
    All lanes : Anti-EEF1E1 Antibody at1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • FC - EEF1E1 Antibody AW5638-U100
    Overlay histogram showing Hela cells stained with AW5638 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5638, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, WB
Primary Accession O43324
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW H=20,16;M=20 KDa
Isotype IgG2b,κ
Antigen Source HUMAN
Additional Information
Gene ID 9521
Antigen Region 1-112 aa
Other Names Eukaryotic translation elongation factor 1 epsilon-1, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Elongation factor p18, Multisynthase complex auxiliary component p18, EEF1E1, AIMP3, P18
Dilution IF~~1:25
WB~~1:2000
FC~~1:25
Target/Specificity This EEF1E1 antibody is generated from a mouse immunized with a recombinant protein of human EEF1E1.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEEF1E1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EEF1E1
Synonyms AIMP3, P18 {ECO:0000303|PubMed:15680327}
Function Positive modulator of ATM response to DNA damage.
Cellular Location Cytoplasm. Cytoplasm, cytosol. Nucleus. Note=Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage
Tissue Location Down-regulated in various cancer tissues.
Research Areas
Citations (0)
citation

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Background

Positive modulator of ATM response to DNA damage.

References

Motegi H.,et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Mao M.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.

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$ 380.00
Cat# AW5638-U100
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